Oak Posted June 8, 2014 Report Share Posted June 8, 2014 so i tried making kinshi, but failed badly. I used king oyster mushroom plugs, but they never seemed to work. However, when i used king oyster mushroom mycellium, it seemed to work better since it contaminated the jars faster. Anyone know the complete step by step guide to make them? I have some plugs left, don't wanna let them go to waste. Thanks. Quote Link to comment Share on other sites More sharing options...
muddymire Posted July 7, 2014 Report Share Posted July 7, 2014 HEY! I've been working on this and it's really difficult. I've used half gallon mason jars with injection ports, quarter in drilled holes covered by micropore tape. I then pressure cooked them at 17psi to 20 psi. Waited overnight for them to cool and then inoculated each with 2ccs of turkey tail. They took! All of them. But growth slowed because the jars are drying out…. Quote Link to comment Share on other sites More sharing options...
LarvaHunter Posted July 7, 2014 Report Share Posted July 7, 2014 Do you think you used too many 1/4" holes and that's why they dried out? Quote Link to comment Share on other sites More sharing options...
muddymire Posted July 7, 2014 Report Share Posted July 7, 2014 Yeah, I used only one hole. I've read that air exchange or gas exchange ( obv. not a mushroom guy) is a precaution. I think it would be a great deal more succesful with 1/16" or 1/32" holes. I added more water with a syringe (not sterilized) I don't think it'll work but I'll let you guys know. I think the best way to do it with plugs is in a sealed clean room (seems difficult), you can see some online (super jank), which is why I chose liquid culture. Using spawn makes the jar colonize faster, and you get more bang for your buck. Try doing it under a super large see through tuperware container, make sure it's clean. Quote Link to comment Share on other sites More sharing options...
LarvaHunter Posted July 8, 2014 Report Share Posted July 8, 2014 You can make a glove box for economy or buy or make a laminar flow hood. I built a laminar flow hood myself with fan and speed control, the most expensive thing was the large hepta filter. Quote Link to comment Share on other sites More sharing options...
muddymire Posted July 9, 2014 Report Share Posted July 9, 2014 laminar flow hood! keeper of information and access. Thank you!!! Quote Link to comment Share on other sites More sharing options...
LarvaHunter Posted July 9, 2014 Report Share Posted July 9, 2014 Lol, no problem. Soon I will post pictures of my equipment,process and progress of growing kinshi. I have cloned mycellium from a kinshi bottle from Japan on some agar. So I completed my first step. Apparently msg is a main ingredient besides bread flour. Quote Link to comment Share on other sites More sharing options...
What Posted September 8, 2014 Report Share Posted September 8, 2014 Have any of you guys read my thread on the topic? LarvaHunter, are you going to post up pics anytime soon? Quote Link to comment Share on other sites More sharing options...
muddymire Posted September 9, 2014 Report Share Posted September 9, 2014 Whoa, that thread! I just was looking through some pages where people where growing carnivorous plants on agar.... I hope to be trying some basics from that thread soon though. Quote Link to comment Share on other sites More sharing options...
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