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Freezing substrate

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I have a few Dhh larvae that are still in the substrate they were in when I bought them. It is time for a sub change but there is a lot of gnats in the current sub. I know you are supposed to mix 20% or so of the old sub with the new. If I cook it will kill the gnats, but also the good bacteria right? Would freezing it kill gnats and mites, but leave the good bacteria ?




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